Abstract

Abstract Flocculating activities of the cell surface substances (FIX-fractions a, b and c) of Nocardia amarae were studied at their minimal concentrations. One of the three substances (FIX-fraction b) proved to exhibit flocculating activity when its concentration was moderately low. The activity was affected by the buffer concentration of the test suspension. Sufficient flocculation was not observed in 1 mM sodium phosphate buffer (pH 6.8), while flocculation occurred in 10 mM and 50 mM buffer. The effects of various salts were also studied. Salts (NaCl, CaCl2, Al2(SO4)3, and FeCl3) stimulated the activity of the flocculant; however, an excessive addition of FeCl3 inhibited flocculation.

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