Abstract
Shoot organogenesis in African Violet was affected by factors including leaf age, wounding and leaf disc orientation. A gradient of regenerative potential was found among individual leaves on a plant. This regenerative potential was not directly proportional to the juvenility of leaf tissues. Wound treatment increased shoot organogenesis only when the wound was in direct contact with the medium, possibly by increasing nutrient and hormone uptake. Leaf disc orientation did not affect shoot number per disc but did determine the position of shoot organogenesis. Reduced gas exchange significantly reduced shoot production. This study also showed that adventitious roots originated from spongy mesophyll cells.
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