Abstract
An unidentified Pseudomonas sp. isolated from pasteurized milk stored at 4C appeared killed by a heat treatment of 55C for 30min, since no colonies formed on trypticase soy agar plates during incubation for 48 to 72 hours. However, when the heated bacteria were held in trypticase soy broth for 48 to 72 hours at 20C or two to three weeks at 4C, some cells recovered ability to grow normally. Heat resistance and recovery were affected by the physiological state of the bacteria and the nature of the heating and the recovery medium. More complex heating media, including milk whey, stabilized the bacteria to heat, and favored recovery. Recovery was also found with five species of Salmonella and is probably a general phenomenon after heating at time and temperature combinations just above those which are apparently lethal.
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