Abstract
1. 1. The reactions of thrombin and plasma were studied. The effect of variations in physical and chemical parameters on clot absorbance and fibrin mass were determined. 2. 2. Variations in pH, ionic strength, temperature and protein concentration affect the spectrophotometric measure of absorbance of a thrombin-plasma clot, but not the mass of fibrin formed. 3. 3. Citrate ion at pH 6.0 greatly retards the development of both absorbance and fibrin formation. 4. 4. Absorbance depends on thrombin concentration and is maximal at 1.0 unit thrombin per milliliter reaction mixture over the pH range 6–8. 5. 5. One unit of thrombin will clot completely (> 95%) 1.17 mg plasma fibrinogen (3.4 nmoles) in 1.0 ml reaction solution at pH 7.35, 38° and ionic strength 0.16. 6. 6. At protein concentrations below 5%, transition from a fine to a coarse clot occurs over the pH range 8.6-6.0. 7. 7. The presence of lipoproteins and other non-clottable globulins affects clot absorbance and measurable fibrin.
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