Factors affecting morphogenetic potential in oilseed rape roots of the Skrzeszowicki and Start cultivars

Abstract

The effect of the origin of root segments, seedling age, growth substances and gelled or liquid media were tested in respect to the morphogenetic potential of rape root segments of Skrzeszowicki (high glucosinolate content) and Start (low glucosinolate content) cultivars. Callus and roots were formed on all root segments after an approximately 2 week growth period; buds were formed after ca. 4 weeks only on segments adjacent to the hypocotyl. Higher concentrations of auxin and cytokinins were required for bud induction. Cultivar differences in the morphogenetic responses of the root segments were found. They were manifested by the more abundant callus formation (BAP+NAA) and more numerous lateral roots and buds (KIN+IBA) on segments from the Skrzeszowicki cultivar than from the Start cultivar.