Abstract
AbstractThe effects of parameters including water content, the amount of celite and the co‐factor regeneration system on reduction of benzaldehyde to benzyl alcohol by yeast alcohol dehydrogenase (YADH) immobilized on celite in non‐conventional media were investigated. Kinetic parameters (apparent) such as Vmax, Km, and kcar for different organic solvents used (butyl acetate, hexane, heptane and i‐octane) were determined. Km values for benzaldehyde increased as the solvent was varied in the following manner: butyl acetate < hexane < i‐octane < heptane. The highest Km value (20.26 mmol/L) was noted in heptane and the lowest (3.24 mmol/L) in butyl acetate. The Vmax value in heptane was more than an order of magnitude higher than that in butyl acetate. Catalytic efficiencies (as expressed by kcar/Km) ranged from 0.703 × 10−4 L/mol · s for hexane to 0.171 × 10−4 L/mol · s for butyl acetate. In general, linear relationships were observed between kcar/Km and the following solvent physico‐chemical constants: dielectric constant logarithm of the saturated molar solubility of water in the organic solvents, log Sw/o, logarithm of partition coefficient of organic solvent in water/octanol two‐phase system, log P, Hildebrand solubility parameter, δ, and solvatochromism of pyridinium‐N‐phenoxide betaine dye, ET(30).
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