Abstract
Microbial-induced calcite precipitation (MICP) technique takes advantage of the metabolic process of bacteria bonding soil particles together with an environmentally-friendly and sustainable manner. In this study, the silt from reaches of Yellow River was used for bio-grouting. The effects of temperature, pH, inoculum size and revolving speed on urease activity were studied by single factor method for Sporosarcina pasteurii. Moreover, the effects of different nutrient concentrations on the stabilization for the typical silt were evaluated. Highest urease activity could be obtained under temperature of 32 °C, inoculum size of 5%, pH of 8 and speed of 180 r/min. Besides, higher concentration of calcium salt could generate more calcium carbonate effectively, and calcium carbonate content can reach to 261mg/g with uniaxial compressive strength of 1.7 MPa.
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