Factor C-LHIH which inhibits the luteinizing hormone from basal release and from synthetic LHRH and studies on purification of FSHRH

Abstract

Data on a new entity which inhibits the release of luteinizing hormone (LH), designated as factor C-LHIH, are presented. In vitro assays were carried out with pituitary glands from female rats. Factor C-LHIH and follicle stimulating hormone releasing hormone (FSHRH) initially fractionated together. FSHRH obscured C-LHIH, and FSH assays guided fractionation. Factor C-LHIH and FSHRH were separated and purified by Sephadex and high pressure liquied chromatography. The resulting fractions of C-LHIH inhibited LH-release at 100-500 ng and did not inhibit thyrotropin releasing hormone-thyroid stimulating hormone response.