Abstract
The effect of prostaglandin (PG) D 2 on neuronal functions was investigated in neuroblastoma × glioma NG108-15 hybrid cells. PGD 2 caused a sustained increase in miniature end-plate potentials (MEPPs) recorded from cultured striated muscle cells which had formed junctions with NG108-15 cells. PGD 2 initially hyperpolarized and then depolarized NG108-15 cells. The time course of depolarization fitted well to the facilitative phase of MEPPs. The same action on synaptic transmission and membrane potentials was detected with PGF 2α but not with PGE 1. PGD 2 (10 −4M) produced a 3-fold increase of adenylate cyclase activity in NG108-15 cell homogenates through its receptors that are distinct from those of PGE 1 and PGI 2. These results show that PGD 2 facilitates MEPP frequency from NG108-15 cells due to depolarization, and suggest that PGD 2 may act as a physiological neuromodulator for synaptic transmission in vivo.
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