Abstract
Targeted radiation of single cells with focussed sub-micron ion beams enables unique investigations in advanced high resolution radiobiology research. Since 2018, the Centre for Ion Beam Applications (CIBA) has operated a dedicated live-cell beam line to study radiobiology on single cells. Recently, we have upgraded this radiobiology beam line with enhanced precision in dose delivery and beam alignment by installing a beam blanking system and stage-mounted exit nozzle, respectively. We have increased efficiency of cell targeting through process automation, combined with exploiting a machine-learning algorithm, Cellpose, for rapid cell recognition to produce a fully automated cell targeting functionality with accurate cell identification. Concordant with further streamlining of our experimental workflows, here we describe the “cell slide”, our novel live-cell chamber assembly, which simplifies and drastically improves throughput and turn-around time in live-cell experiments. Cell slide has been specifically developed to support live-cell radiobiology applications on microbeam platforms.
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