Facilitating effects of plant hormones on biomass production and nutrients removal by Tetraselmis cordiformis for advanced sewage treatment and its mechanism

Abstract

Advanced sewage treatment by microalgae is regarded as a promising method for addressing eutrophication. To improve sewage treatment, three kinds of plant hormones including auxin (indole-3-acetic acid, IAA), cytokinin (Zeatin), and brassinosteroid, were chosen to measure the influence of plant hormones on nitrogen and phosphorus removal by Tetraselmis cordiformis and to analyze their mechanisms, including photosynthesis, nutrient metabolism, and gene transcription. The results indicated that the maximal removal efficiencies of total nitrogen and phosphate by T. cordiformis were elevated by the plant hormones by 184.3% and 53.2%, respectively. The chlorophyll a content was increased by 1.1 times by the plant hormones in comparison with the control. Moreover, after being stimulated by plant hormones, the activities of nitrate reductase (NR) and glutamine synthetase (GS) increased by 90.4% and 82.1%, respectively, in comparison with the control. Supplementation with plant hormones also significantly elevated the mRNA expression level of GS-related gene by 30.9%. This study demonstrated that plant hormones could significantly promote the nutrient removal of microalgae for sewage treatment in artificial laboratory conditions and provided theoretical support for its further practical full-scale application under variable conditions.