Facile-synthesis and characterization of cobalt oxide (Co3O4) nanoparticles by using Arishta leaves assisted biological molecules and its antibacterial and antifungal activities

Abstract

A novel biosynthesis of cobalt oxide nanoparticles (Co3O4 NPs) attains using Arishta leaves (Neem leaves) extract at disparate annealing temperature, (Untreated (RT), 200 °C, 400 °C, 600 °C, and 800 °C) was analysed. The structural and morphological analysis has been carried out by using different characterization techniques. The characterization techniques such as X ray diffraction and scanning electron microscopy reveals that the Arishta leaves extract act as a fundamental, oxidation-reductant in addition to mediated preserving compound. Also the transparent and nonporous pyramid like crystalline structure of Co3O4 is confirmed. The antibacterial and antifungal behaviour of synthesized nanoparticles was analysed against Staphylococcus aureus, Streptococcus mutans, Klebsilla pneumonia and Escherichia coli as well as Aspergillus species of A. flavus and A. Niger fungi by agar disc diffusion process. Because of the pH, reaction temperature, reaction time, and extract clustering during the synthesis of Co3O4 NPs, the synthesized neem Co3O4 NPs have a strong antibacterial and antifungal activity. These findings show that the leaf extract may effectively operate as a dynamic response medium for the formation of Co3O4 NPs, and that it can be used in a variety of biomedical by-products and nano devices due to its low cost and high efficiency.