Abstract
AbstractThe bacterial communities (Gram-negative and Gram-positive) form the biofilms which oppose the mode of action of antibiotics and affecting the immune system of the human. These chronic infections related to biofilm are always hard to be cured because of their inherent resistance to both antimicrobial agents and host defense. The present study is devoted to the synthesis of silver nanoparticles (AgNPs) using aqueous extracts ofAverrhoa bilimbileaf and Plum fruit (Prunus bokharensis) and its inhibitory effect onStaphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, andSalmonella typhi. Here, AgNPs) were successfully prepared by green synthesis method obtained by the reduction of silver nitrate into silver ions. The synthesized AgNPs were characterized by UV-Vis Spectroscopy, XRD, FTIR, FESEM-EDAX and DLS analysis. The antimicrobial effects of the two extract were evaluated by the disk diffusion method. The green synthesized AgNPs exhibited good antibacterial activity against both Gram-negative and Gram-positive. They were also analyzed for their significant antibacterial activities against all the test cultures exposed whereasEscherichia coliandSalmonella typhitopped among other test cultures. The anti-proliferative activity of phytochemical mediated synthesis of Ag NPs was investigated for their cytotoxicity in Vero and Human epidermoid larynx carcinoma cell lines (HEp-2) based on their viability using MTT assay. The present study also represents the synergistic bioactivity of silver nanoparticles using in vitro models.
Highlights
IntroductionAgNAP,s)Aw∗eirsessauicd- to be self-dual whenever there exists an automorphism of the endom cessfully prepared by green synthesis methosdwoabptasinAedanbdy A∗
The extract of Averrhoa bilimbi leaf and Plum fruit were mixed and incubated with AgNO3 solution, change in color of the solution from pale green to dark brown indicated the visible reduction of leaf and fruit extracts arbitrated synthesis of AgNPs
Similar results have been reported in Earlier studies on the synthesis of AgNPs synthesized by A. bilimbi leaf extract report similar outcomes confirming the complete transformation of and AgNO3 to AgNPs using Averrhoa bilimbi leaf and Plum fruit extracts [22]
Summary
AgNAP,s)Aw∗eirsessauicd- to be self-dual whenever there exists an automorphism of the endom cessfully prepared by green synthesis methosdwoabptasinAedanbdy A∗. In this case such an automorphism is unique, and called the dua the reduction of silver nitrate into silver ions. ThTehseynlittheer-atu1re cIonnttraoinds mucantyioexnamples of self-dual Leonard pairs. Sized AgNPs were characterized by UV-Vis aStpeedctwroitshcoapny,irreducible module for the Terwilliger algebra of the hypercube TLheeoannatridmipcaroir- ofAKnratiwbitocthicosuakretyppreom(siesein[g10c,aDnedidnaitteioinn 6t.h1e]);tr(eiiait)mthenetLoefonard pair asso bial effects of the diffusion method. TLheeoannatridmipcaroir- ofAKnratiwbitocthicosuakretyppreom(siesein[g10c,aDnedidnaitteioinn 6t.h1e]);tr(eiiait)mthenetLoefonard pair asso bial effects of the diffusion method. good antibacterial
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