Abstract
Dopamine acts as a neurotransmitter to regulate a variety of physiological functions of the central nervous system. Thus, the fabrication of electrochemical active nanomaterials for sensitive dopamine detection is extremely important for human health. Herein, we constructed a highly efficient dopamine nonenzymatic biosensor using eggshell membrane (ESM) as a 3D network-like carrier-loaded Au and CeO2 nanocomposites. This approach has led to the uniform distribution of CeO2 and Au nanoparticles on the surface of ESM. The structure and properties of the as-prepared ESM templated Au/CeO2 (ESM-AC) nanocomposites were characterized. The electrochemical properties of non-enzymatic oxidation of dopamine by ESM-AC electrode were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The detection limit of the ESM-AC modified electrode for dopamine is 0.26 μM with a linear range from 0.1 to 10 mM. The ESM-AC-modified electrode performs a higher catalytic activity for dopamine electrocatalytic oxidation than that ESM-templated CeO2 (ESM-C) electrode, which is mainly due to the unique structure of ESM and more active sites provided from Au. Collectively, this biological waste-ESM provides a cheap and unique template for the preparation of 3D network-like nanostructures and expands the application in electrochemical dopamine detection.Graphical abstractESM-AC nanocomposites prepared from biological waste was successfully modified on the surface of glassy carbon electrode and a dopamine-based electrochemical biosensor was constructed.
Highlights
Dopamine is a neurotransmitter to help cells transmit pulsed chemicals
In the HAADF characterization of eggshell membrane (ESM)-AC nanomaterials (Fig. 1(d)), CeO2 was uniformly distributed on the surface of ESM, and the Au nanoparticles were uniformly distributed in the material mainly at a particle size of 60-90 nm
To further verify the properties of the pore structure, we measured the finer surface properties of the ESM-templated CeO2 (ESM-C) and ESM templated Au/CeO2 (ESM-AC) nanocomposites by measuring the specific surface area based on the N2 adsorption−desorption isotherms using Brunauer−Emmett−Teller (BET) method and the Barrett −Joyner−Halenda (BJH) method based on the adsorption curve to obtain the corresponding pore size distribution (Fig. 1(e, f))
Summary
Scientific research has shown that various addictive behaviors are closely related to dopamine [1]. The content of dopamine in the body is directly related to life and health, so the establishment of rapid, efficient, and extensive dopamine detection method is of great importance in practical applications [2]. The commonly used methods for determination of dopamine include fluorescence analysis [3, 4]. Cerium oxide is a non-inert carrier of a commonly used catalyst which simultaneously exists in Ce3+ and Ce4+ oxidation states on the lattice surface and is currently used in many fields such as analysis detection, drug delivery, and photocatalysis [15]. In the past few years, great progress has been made in scientific research around CeO2 [16].
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