Facile synthesis of boronic acid-functionalized magnetic nanoparticles for efficient dopamine extraction

Jeong Keun Kook,Do-Yeong Koh,Viet-Duc Phung,Sang-Wha Lee

doi:10.1186/s40580-019-0200-7

Jeong Keun Kook, Do-Yeong Koh + Show 2 more

Open Access

https://doi.org/10.1186/s40580-019-0200-7

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Journal: Nano convergence	Publication Date: Sep 2, 2019
Citations: 22	License type: open-access

Affiliation: Gachon University, Duy Tan University

Abstract

Because dopamine (DA) is one of the most critical neurotransmitters that influence a wide variety of motivated human behaviors, it is necessary to develop a facile diagnostic tool that can quantify the physiological level. In this study, core–shell magnetic silica nanoparticles (Fe3O4@SiO2) were prepared using a modified sol–gel reaction. The Fe3O4@SiO2 were functionalized using 3-aminophenylboronic acid (APBA) via a facile and rapid synthetic route, hereafter referred to as Fe3O4@SiO2@APBA The resultant Fe3O4@SiO2@APBA not only adsorbed DA molecules, but also were easily separated from solution using a simple magnetic manipulation. The adsorbed amounts of DA by the Fe3O4@SiO2@APBA were quantified by measuring the changes in fluorescence intensity of polydopamine (at 463 nm) originated from the self-polymerized DA remained in the supernatant before and after the adsorption process. The Fe3O4@SiO2@APBA exhibited two-stage adsorption behavior for DA, and the maximal adsorption capacity was 108.46 μg/g at pH 8.5. Our particle system demonstrated the potential application for extracting compounds with cis-diols (including catechol amines) from the biological fluid.

Highlights

Dopamine (DA) is one of the most important catecholamine neurotransmitters, which plays major roles in central nervous system processes, such as rewardingmotivated behavior in the brain, regulating exercise, and controlling the release of various hormones [1,2,3,4]
The residual DA in the supernatant was quantified based on the fluorescence intensity of PDA, which formed from the self-polymerized DA under alkaline conditions
4 Conclusions In this study, the F e3O4@SiO2@aminophenylboronic acid (APBA) were facilely prepared for the efficient adsorption of DA molecules

Summary

Introduction

Dopamine (DA) is one of the most important catecholamine neurotransmitters, which plays major roles in central nervous system processes, such as rewardingmotivated behavior in the brain, regulating exercise, and controlling the release of various hormones [1,2,3,4]. Since the DA system plays a key role in several medical conditions, such as Parkinson’s disease, Segawa disease, schizophrenia, and attention deficit hyperactivity disorder, DA concentration in biological fluids is used as important indicator for early disease diagnosis [5, 6]. It is essential to separate target molecules from coexisting interfering components for accurate analysis. Solid-phase extraction (SPE) is the preferred method for concentrating and purifying small amounts of analytes from biological fluids [15, 16]. Magnetic solid-phase extraction (MSPE) is a new process for pre-concentrating target analytes using magnetic adsorbents [17]. The MSPE process has been applied in a wide range of fields for the separation of various materials because the adsorbent material does not need to be packed into any type of device, unlike traditional SPE [18, 19]. The silica surface is activated and can anchor various functional groups because of its abundant Si–OH groups [19, 20]

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Conclusion