Facile Synthesis and Characterization of Eco-friendly Collagen based silver Nanoparticles and its Biological Activities

Abstract

Abstract: For the past few decades, the natural polymer Type-I Collagen becomes more attention due to its biomedical applications like scaffold preparation, tissue engineering and nanomaterial production. Fish skin bio waste has been recognized as economical, widely accepted alternative to Collagen derived sources. This present study deals with the effective use of underutilized bio waste resource. Collagen was extracted and confirmed as Type-I Collagen from the fish skin bio waste. After that the AgNPs were synthesized from the Collagen. The Protein profile of extracted Collagen was analysed in the SDS-PAGE confirms the type of Collagen. The morphology of type-I collagen was revealed under SEM. The silver nanoparticle was synthesized by using silver nitrate as a reducing and Capping agent for the Collagen along with sodium borohydride (NaBH4). The results of UV-Vis Spectroscopy confirmed the formation of AgNPs by showing a sharp absorption peak at 418nm. FTIR spectral peak shows the presence of alkynes, carboxylic acids and anhydrides compound which binds with Ag and also reduces and capped on Ag ions for the conversion of Ag+ into AgNPs. The DLS and zeta potential results positively shows the stability of synthesized nanoparticles. The morphology of the resulted AgNPs exhibited spherical shape with less than 50nm size under SEM. The antimicrobial activity possess the suitable material for the biomedical applications, the Cytotoxicity of the Collagen derived AgNPs were evaluated under the MG-63 cell line and the toxicity results having an Dose dependent manner. Key words: Collagen , AgNPs, Characterizations , Antimicrobial activity, MTT.