Facile, sustainable, and innovative inclusion of a highly fluorescent isoindole-sulfonate-based probe for the quantification of fluvoxamine, a CNS antidepressant drug; comprehensive method sustainability evaluation

Abstract

The suggested study employs a specific technique to ensure the sustainability and ecological friendliness of an analyte evaluation procedure. The utilization of various recently developed methodologies as potential indicators of ecological sustainability and feasibility necessitates attention and consideration. The established analytical methodology employs a novel reagent mixture composed of phthalic dialdehyde and sulfite ion. This reagent is used to create a new fluorescent core that can be monitored using fluorimetric techniques at a wavelength of 385 nm after being excited at 320 nm. Condensation of the amino group of fluvoxamine can occur in a pH-controlled solution containing phthalaldehyde/sulfite. The present investigation presents a new, environmentally sustainable, and accurate fluorometric technique for identifying the medication. A linear relationship was detected for fluvoxamine over the 0.06–1.50 μg mL−1 concentration range. The method's validation and quantum yield determination were assessed following the International Council for Harmonization's (I.C.H.) established guidelines. The results exhibit remarkable precision and accuracy, consistent with the different methodologies published in the literature. Subsequently, an assessment was conducted to evaluate the environmental sustainability of the suggested technique, employing a range of contemporary indicators for determining environmental safety. The WAC concept, which combines ecological and operational elements and makes use of the Green/Red/Blue (RGB 12) scheme, interestingly identifies the current analytical method as white. Finally, BAGI, a novel tool for evaluating the method's applicability, was used in this study and showed that the planned method achieved a high applicability score.