Abstract
The purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, the purified chlorophylls can be converted to relatively pure chlorophyllides facilely. The results show that higher purity of chlorophyllides could be obtained when purified chlorophylls (ethanol-hexane extract) was used as starting materials than that of crude chlorophylls (ethanol-only extract). In biocompatibility test, the results showed that the prepared chlorophyllides can be applied as biomaterials. When the prepared chlorophyllides were applied to anticancer tests, they were active both in MCF7 and MDA-MB-231 (multidrug resistant breast cancer cells) cell lines. In addition, the results suggested that the prepared chlorophyllides could be a potential candidate of combination therapy with doxorubicin to breast cancers.
Highlights
Chlorophyllides were first named by Willstatter and Stoll in 1911 [1]
Purified chlorophyllides were obtained in the aqueous phase when the purified chlorophylls were hydrolyzed with chlorophyllase
The results revealed that the purity of chlorophyllide a and chlorophyllides increased by 43.5% and 14.55%, respectively
Summary
Chlorophyllides were first named by Willstatter and Stoll in 1911 [1]. Chlorophyllides are a phytol-free form of chlorophylls. Chlorophyllides exist in cyanobacteria, green algae and plants, while bacteriochlorophyllides exist in phototrophic bacteria [2]. Chlorophyllides in the photosystems of plants are of two types. Chlorophyllide a and chlorophyllide b vary slightly in the chemical structure of the porphyrin ring, having molecular formulae of C35H34MgN4O5 and C35H32MgN4O6, respectively. Chlorophyllides have been used for diverse applications. The antigrowth ability of Staphylococcus aureus [3], photodynamic activity as photosensitizers in photodynamic inactivation of Staphylococcus aureus [4], antimicrobial
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