Abstract
In this article, a Fe3O4@MOF core-shell microsphere has been successfully prepared by growing MIL-100(Fe), three dimensional (3D) metal–organic frameworks (MOFs) onto Fe3O4 nanoparticles. The resultant composite microsphere exhibited both magnetic characteristics and large specific surface area, making them excellent candidates for enzyme immobilization. Candida rugosa lipase was immobilized as a model enzyme on core-shell microspheres. The results showed that the immobilized enzyme for hydrolysis of olive oil retained >65% of its initial activity at 65°C over 6h; and the residual activity still remained about 60% of the initial activity after the 10th catalysis run. Therefore, such MOF based core-shell magnetic composite microspheres showed promising applications as an enzyme immobilization support.
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