Abstract
The increase of homocysteine (Hcy) is closely related with the development of many diseases. Nevertheless, the effective detection of Hcy remains challenging due to the lack of a simple, specific,and cost-efficient method. Herein, an electrochemical biosensor based on Hcy aptamer/Au nano-particles/graphene sponge (Aptamer/Au NPs/GS) was designed for the detection of homocysteine. The biosensor employed methyleneblue(MB) as a redox indicator. Due to the change of aptamer conformation, the adsorbed MB was released from aptamer, resulting in the decrease of electrochemical response. In the range of 1–100 μM, the concentration of Hcy had a good linear relationship with the relative redox peak current (ΔI) of MB and the lowest detection limit was 1 μM. The experimental results demonstrated the aptasensor had good selectivity and reproducibility. In real serum sample, the aptasensor showed an acceptable recovery rate indicating its potential applications in the preliminary detection of various diseases associated with Hcy.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
