Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Hassan Bardania,Seyyed Ebrahim Moosavifard,Mohamad Hassan Fouani,Zeinab Salehpour,Danesh Javeshghani,Bita Darabian,Gunna Christiansen,Ali Mousavizadeh,Mohsen Alipour,Reza Mahmoudi,Hamed Bagheri,Majid Kowsari,Seyed Sajjad Khoramrooz,Mohammad Akrami

doi:10.1038/s41598-020-63032-5

Abstract

To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.

Highlights

To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes
We report the synthesis of AgNPs, using Teucrium polium’s (T. polium) hydro-alcoholic extract
The designed bi-functional particle constitutes the main reinforcement for poly lactic acid/poly ethylene glycol (PLA/polyethylene glycol (PEG))/Ag nano composite to improve the wound healing features of poly lactic acid (PLA)/PEG co-polymer

Summary

Introduction

To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Nanofibers prepared from renewable polymers, due to their extremely high specific surface area, porosity and excellent pore interconnectivity, excellent performances in cell adhesion, migration, proliferation, differentiation, and the analogous physical properties of the extracellular matrix (ECM) have attracted considerable attention over the past two decades[4] Such characteristics allow structured nanofibers to promote hemostasis without the use of a hemostatic agent, maintain an adequately humid environment for the wound by facilitating oxygen permeation and allowing fluid accumulation, protect the wound effectively against bacterial penetration, and be functionalized with therapeutic compounds[5]. In its ionic or nanocrystalline form, silver has been exploited as an antimicrobial agent against a broad spectrum of pathogenic micro-organisms; a detailed history of silver usage has been well documented in literature[12]

Methods

Results

Conclusion