Facile one-pot green solvent synergized fluorescence reaction for determination of doxorubicin in presence of paclitaxel; co-administered drug, application to stability study and analysis in bulk, vial and biological fluids

Abstract

A green, fast, simple, economical, ultra-sensitive and accurate spectrofluorimetric method was described in the article for the estimation of Doxorubicin hydrochloride (DOX), a type II topoisomerase enzyme inhibitor either alone and in the presence of co- administered drug; paclitaxel as adjuvant chemotherapy combination in bulk, pharmaceutical dosage form and biological fluids. The proposed method was based on studying the fluorescence demeanor of DOX in different organized media and solvents. The intrinsic fluorescence was found to be greatly enhanced in acidic ethanolic solution. The maximum intensity of fluorescence was observed at 590 nm after excitation at 475 nm. Different experimental parameters were examined and adjusted involving various organized media, solvent, and pH. The methods was validated according to ICH guidelines. The linearity of the method was accomplished in the range of 7 × 10−4–4.0 µg ml−1. The values of LOD and LOQ were 0.2 and 0.6 ng ml−1 respectively. The present method is extremely sensitive enough for determination of traces of the drug in various biological fluids with excellent % recovery. The proposed method was extended to investigate the stability of DOX following various induced degradation protocols including: acidic, alkaline, oxidative and photolytic. In addition, the kinetics of the degradation of DOX was investigated and the apparent first-order rate constant, half-life times and Quantum yield were calculated.