Abstract
The main purpose of this manuscript is to introduce a facile light microscopy methodology to visualize plant roots filaments. In a previous manuscript in vitro experiments on freshly plucked human hair roots documented the commonly used antibiotic Tetracycline (TE) deleterious effect on soft tissue, severe enough to allow for visualization of an underlying filamentous skeleton. In this manuscript, TE was also evaluated in a similar fashion of in vitro experiments, this time aerial plant roots were immersed in liquid Tetracycline. Images and video recordings are presented where plant aerial root tissue cells appeared to interact with Tetracycline, thus allowing for exposure of an underlying filamentous network. These filaments were documented undergoing biosorption of Tetracycline, thus indicating a probable cellulose base. It is emphasized that a literature search showed similar, albeit visually different displays of roots filaments obtained by using a Scanning Electron Microscopy. The method herein introduced could be an adjunct to existing established methodology in root function research. Two salient advantages are identified, firstly that the essential minimal material and equipment is limited to a light microscope, glass slides, chosen biological material, water and powder Tetracycline. Secondly, the speed in obtaining results would offer researchers a preliminary or perhaps a final correct conclusion.
Highlights
The deleterious effect of Tetracycline on the human hair follicle soft tissue was recently demonstrated in vitro [1]
To show the results of the effect of liquid Tetracycline” (LT) on plant root tissue, we need to refer the reader to an image showing the effect of LT in human soft tissue tissue of the human hair follicle a.k.a. root (1) and Figure 2 below
Since the antibiotic Tetracycline and its derivatives have been reported to impact on the physiology and biochemistry in tissues of plants and animals [6], it could be stated that it has a deleterious impact on plant and animal cells, to the point of sort of eliminating barriers in light microscopy fields, allowing for the visualization of the repetitive patterns introduced (Figs 4,5 plus video recording) supporting this principle
Summary
The deleterious effect of Tetracycline on the human hair follicle soft tissue was recently demonstrated in vitro [1]. The adhesive property of TE was proposed as one factor in damaging the human follicle’s metabolism, as well as a myriad of soft tissue cells. The present manuscript introduces a deleterious effect of liquid TE to aerial plant root tip tissue. Fresh harvested orchid hanging aerial root tips were exposed to liquid TE by via a previously describe technique dubbed a Single Slide Preparation (SSP) [2]. The images obtained show a damaging effect of TE on external root epidermal and internal trichoblats cells, documented are detailed images of a continuous filamentous veil seen covering the interior tissue. The outer epidermal cells failed to exhibit a filamentous covering network
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