Abstract
BackgroundImmobilization of lipase on appropriate solid supports is one way to improve their stability and activity, and can be reused for large scale applications. A sample, cost- effective and high loading capacity method is still challenging.ResultsA facile method of lipase immobilization was developed in this study, by the use of polydopamine coated magnetic nanoparticles (PD-MNPs). Under optimal conditions, 73.9% of the available lipase was immobilized on PD-MNPs, yielding a lipase loading capacity as high as 429 mg/g. Enzyme assays revealed that lipase immobilized on PD-MNPs displayed enhanced pH and thermal stability compared to free lipase. Furthermore, lipase immobilized on PD-MNPs was easily isolated from the reaction medium by magnetic separation and retained more than 70% of initial activity after 21 repeated cycles of enzyme reaction followed by magnetic separation.ConclusionsImmobilization of enzyme onto magnetic iron oxide nanoparticles via poly-dopamine film is economical, facile and efficient.
Highlights
Immobilization of lipase on appropriate solid supports is one way to improve their stability and activity, and can be reused for large scale applications
We present a facile, biomimetic approach to immobilize lipases onto iron oxide magnetic nanoparticles (MNPs) surfaces modified with polydopamine, an in-situ formed coating inspired by the adhesive proteins secreted by marine mussels [13]
Our results demonstrate that polydopamine film on MNPs (PD-MNPs) exhibit high efficiency for lipase immobilization under aqueous conditions, and the enzyme retains high activity after many cycles of magnetic separation and reuse
Summary
Immobilization of lipase on appropriate solid supports is one way to improve their stability and activity, and can be reused for large scale applications. Typical strategies for immobilizing lipase onto MNPs rely on surface grafting via low molecular weight linkers or polymers containing amino or epoxy functional groups to which lipases are reacted via covalent conjugation methods [1,11]. Using such methods, the maximum reported loading capacity of lipase on nanoparticles is approximately 130 mg/g, using a complex methodology [1]. Despite numerous reported approaches for immobilization of lipases on magnetic nanoparticles, there is still the need for simple, cost-effective and high loading capacity methods
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