Facile fabrication of electrospun regenerated cellulose nanofiber scaffold for potential bone-tissue engineering application

Abstract

In this study, cellulose acetate (CA) solutions (9–15% w/v) prepared in acetone-water (80:20 & 90:10 v/v) system were subjected to electrospinning for fabricating non-woven nanofibrous CA scaffolds (CAS) with average fiber diameters from 300 to 600 nm. Further, regenerated cellulose scaffold (RCS) was obtained by deacetylation of electrospun CAS in alkaline media for varying time periods to find the ideal time required for complete deacetylation. Following deacetylation, RCS was subjected to varying temperatures (60 °C, 80 °C) to observe the possible positive effect of heat treatment on the improvement of mechanical strength. The RCS was characterized using ATR FTIR, SEM for studying its surface chemistry and morphology along with other physio-chemical characterizations such as micro-tensile strength, swelling property, porosity, degradation rate in acidic conditions. The results were analyzed and co-related with variation of composition in solvent system, deacetylation time and heat treatment temperatures to determine the optimal fabricating conditions for RCS. In vitro studies using MC3T3-E1 osteoblast cells were also conducted on the selected RCS samples to evaluate cell adhesion and cell proliferation using SEM and MTT assay analysis. The primary results indicate positive outcome regarding the viability of RCS as potential biomaterial for bone-tissue engineering.