Abstract
A novel, facile DNA detection method based on aggregation/ disaggregation of an AIE (aggregation induced emission) dye- labeled peptide nucleic acid (PNA) probe was developed. This PNA probe plays the role of both target recognition and signal emission, thus it can quantify the target DNA without requiring additives such as intercalators and can distinguish the target from other sequences.
Highlights
In recent years, aggregation-induced emission (AIE) dyes have attracted attention as smart materials in the fields of luminescent materials [1], imaging [2] and sensors [3]
We describe the detection of telomeric DNA by fluorescence measurements based on the AIE-peptide nucleic acid (PNA) probe method, where the AIE unit is a tetraphenylethylene (TPE) derivative (Figure 1), and investigate the operating principles of this DNA detection system
Synthesized and purchased DNA and PNA samples were dissolved in MilliQ water, and the concentrations of these solutions were determined from the absorbance at 260 nm
Summary
Aggregation-induced emission (AIE) dyes have attracted attention as smart materials in the fields of luminescent materials [1], imaging [2] and sensors [3]. When AIE–PNA is hybridized with telomeric DNA, the AIE dye moiety will be dispersed in solution and fluorescence quenching will be induced (Signal-OFF). The AIE-PNA probe is expected to work as an alternative method for the detection of telomeric DNA, without requiring an intercalator such as EB.
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