Abstract
Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
Highlights
Lipid vesicles or liposomes are 3D structures made from a bilayer of phospholipid molecules enclosing an aqueous compartment
Giant vesicles can be formulated by adding an aqueous buffer to a dried thin film of lipids [2,3]
We have presented presented aa rapid rapid formulation formulation technique technique for for monodisperse monodisperse giant
Summary
Lipid vesicles or liposomes are 3D structures made from a bilayer of phospholipid molecules enclosing an aqueous compartment. Giant vesicles can be formulated by adding an aqueous buffer to a dried thin film of lipids [2,3] To accelerate this process, the film can be placed between two conductive plates and an applied alternating electrical field will promote the swelling of the lipid film into liposomes [4]. The speed of liposome formation is hereby highly increased compared to conventional hydration techniques and the method accommodates for a large range of lipid compositions and buffer conditions. It enables the efficient encapsulation of numerous biomolecules [9] including proteins [10]. Same bead or on different beads) can be combined into a hybrid vesicle
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