Abstract
Magnetic lipase was prepared by a facial and cost-effective method. Lipase from Pseudomonase cepacia was covalently linked to Fe3O4 nanoparticles, which were produced by co-precipitating Fe2+and Fe3+ ions in ammonia solution and subsequent coating with 3,4-dihydroxylaldehyde. Magnetic Fe3O4 nanoparticles modified with 3,4-dihydroxylaldehyde bonded lipase efficiently(47 mg/g). The resulting magnetic-lipase is active(0.68 μmol·min–1·mg–1) and enantioselective(e.e. of the product >98%) in the hydrolysis of racemic 1-phenylethyl acetate. Furthermore, as a heterogeneous catalyst, the magnetic-lipase can be magnetically recycled, and a retained activity of 48% was obtained even after 6 cycles.
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