Abstract
Objective Human aortic valve interstitial cells (HAVICs) were seeded onto decellularized valves in order to fabricate tissue engineering heart valves in a bioreactor system.Methods Decellularized valves were cross-linked by polyethylene glycol.PEGylated decellularized valves were covalently modified by GRGDSPC peptides.The modified effect of GRGDSPC peptides was determined by using immunofluorescence.HAVICs were seeded onto PEGylated decellularized valves modified by GRGDSPC peptides.Then the valves were sutured into a bioreactor system.Cell adhesion was tested respectively after 2,4 and 8 h.After 10 days of culture,hematoxylin-eosin staining,scanning electron microscopy and DNA content test were performed.Results Immunofluorescence showed that PEGylated decellularized valves could be effectively modified by GRGDSPC peptides through covalent bonding.As compared with decellularized valves,PEGylated decellularized valves modified by GRGDSPC peptides significantly promoted the adhesion of HAVICs.Number of adherent cells after 2,4 and 8 h was respectively (4.98 ±0.46) × 107/L vs.(3.35 ±0.15) ×107/L,(5.71 ±0.29)× 107/L vs.(3.55 ±0.18) ×107/L,(5.97 ±0.45) ×107/L vs.(3.62 ±0.18) × 107/L,P ＜0.05.The DNA content of PEGylated decellularized valves modified by GRGDSPC peptides was significantly higher than that of decellularized valves [(25.98 ± 2.45) μg/valve vs.(19.61 ±1.94) μg/valve,P ＜ 0.05].Conclusion Modification of PEGylated decellutarized valves by GRGDSPC peptides can promote the adhesion of cells seeded on valves in a bioreactor system. Key words: Decellularized valves; Polyethylene glycol; Bioreactor; Adhesion
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