Abstract
The fabrication of three-dimensional scaffolds has attracted more attention in tissue engineering. The purpose of this study is to explore a new method for the fabrication of three-dimensional micro-nanofiber structures by combining solution blow spinning and rotating collector. In this study, we successfully fabricated fibers with a minimum diameter of 200 nm and a three-dimensional structure with a maximum porosity of 89.9%. At the same time, the influence of various parameters such as the solvent volatility, the shape of the collector, the feed rate of the solution and the applied gas pressure were studied. It is found that solvent volatility has large effect on the formation of the three-dimensional shape of the structure. The shape of the collector affects the porosity and fiber distribution of the three-dimensional structure. The fiber diameter and fiber uniformity can be controlled by adjusting the solution feed rate and the applied gas pressure. It is feasible to fabricate high-quality three-dimensional micro-nanofiber structure by this new method, which has great potential in tissue engineering.
Highlights
Three-dimensional (3D) micro-nanofiber scaffolds are becoming more important in current tissue engineering research
The 3D shape of the micro-nanofiber structure gives cells the opportunity to vertically proliferate compared to a two-dimensional (2D) membrane, and can grow into a 3D shaped tissue like bone[1,2] or liver[3] instead of 2D shape like skin.[4,5]
This study provided good guideline for fabricating 3D shape scaffolds by utilizing novel homemade device and method
Summary
Three-dimensional (3D) micro-nanofiber scaffolds are becoming more important in current tissue engineering research. The 3D shape of the micro-nanofiber structure gives cells the opportunity to vertically proliferate compared to a two-dimensional (2D) membrane, and can grow into a 3D shaped tissue like bone[1,2] or liver[3] instead of 2D shape like skin.[4,5] On the other hand, porosity is one of the most important properties of micro-nanofiber scaffolds. High porosity ensures cell migration and nutrient transport.[6] In general, ideal tissue-engineered scaffolds should have 3D shape and high porosity to enhance cell attachment, cell-matrix interactions and proliferation.[7]. In the past few decades, researchers have developed several methods to fabricate 3D structures, including electrospinning,8,9 3D printing,[10] light curing[11] and so on. Bryan[15] et al obtained a cotton ball-like electrospun scaffold by designing a spherical dish
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
