Fabrication of solid lipid nanoparticles containing vitexin protects dopaminergic neurons against 6-hydroxydopamine induced Parkinson's disease model via altered the genetic backgrounds

Abstract

Vitexin is a flavonoid which obtained from Crataegus pinnatifida Bunge. Further, its neuroprotective effects are examined in PD model. The motive of the current study was to fabricate vitexin loaded solid lipid nanoparticles and evaluate the effect against 6- hidroxidopamine (6-OHDA) induced parkinson's disease animal model. Intracerebroventricular injection of 6-OHDA was used for induction the PD and mice were treated with the said drug loaded nanocarrier for 28 days. In our study, hot melt emulsification and ultrasonication method was employed for the preparation of Vitexin loaded nanolipoidal carrier. Further, the enzymatic activities incudes Catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) glutathione peroxidase (GPx), glutathione S-transferase (GST), level of reactive oxygen species, total reactive antioxidant dopamine and its metabolite homovanilic and 3, 4-dihydroxyphenylacetic acid, were determined in the striatum. Furthermore, the behavioural parameters including locomotor, memory and depressive were estimated. The Vitexin loaded solid lipid nanoparticles therapeutics in 6-OHDA induced Parkinson's, results clearly showed improvement in total reactive antioxidant, CAT, GPx, Dopamine and its metabolite level in striatum of mice. We also observed that reactive oxygen species in the striatum were diminished by solid lipid nanoparticles of Vitexin. Furthermore, the said formulation is well efficient in averting the memory destruction in Morris water maze test as well as depressive like behaviour in tail suspension test. Vitexin loaded solid lipid nanoparticles founds protective effects in neurodegeneration on 6-OHDA induced neurotoxicity in mice. In features, this could be alternative effective therapeutic approach for the management of PD.