Fabrication of Size-Controllable Nanofluidic Channels by Nanoimprinting and Its Application for DNA Stretching

Abstract

We report a new approach to greatly simplify the fabrication of nanofluidic channels with well-controlled dimensions. It is achieved by imprinting a channel template into a thin polymer film cast on a glass cover slip in a single step, offering a much higher throughput than previous methods. We demonstrated effective DNA stretching in these nanochannels. This method provides a simple and practical solution for low-cost fabrication of nanofluidic channels, which may serve as a useful tool for chemical analysis system on the nanoscale.