Abstract
Single-cell microarrays may play an important role in understanding the heterogeneity of cell populations. A hydrogel-coated micropatterned surface was prepared to capture single cells from a mixture of cells and detect their mitochondrial activity using real-time fluorescence analysis. The double-layered hydrogel micropatterns were functionalized with RGD-containing peptides using a photomask. We were able to control the number of cells immobilized on each spot of the micropattern by controlling the size of the spots. Single cells were properly arranged on the peptide-functionalized spot with aligned squares (20 μm × 20 μm), with 68% frequency of single cells. In the single-cell micropattern, a mitochondria-targetable switch-on-type of probe was employed to discriminate between the types of cells and exhibit heterogeneous cell behavior in real-time. This single cell detection technology allows for the analysis of cell function and gene expression in various environments.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.