Abstract
A simple chemical-etching approach is used to prepare the silicon carbide quantum dots (QDs). The raw materials of silicon carbide (SiC) with homogeneous nanoparticles fabricated via self-propagating combustion synthesis are corroded in mixture etchants of nitric and hydrofluoric acid. After sonication and chromatography in the ultra-gravity field for the etched products, aqueous solution with QDs can be obtained. The microstructure evolution of raw particles and optical properties of QDs were measured. Different organophilic groups on the surface like carboxyl, oxygroup, and hyfroxy were produced in the process of etching. Fluorescent labeling and imaging for living cells of Aureobasidium pulluans were investigated. The results indicated that SiC QDs were not cytotoxic and could stably label due to the conjugation between organophilic groups of QDs and specific protein of cells, it can be utilized for fluorescent imaging and tracking cells with in vivo and long-term-distance. Moreover, mechanism and specificity of mark were also analyzed.
Highlights
Quantum dots (QDs) have attracted considerable interest over the past two decades due to their remarkable luminescent properties which are known to offer several unique advantages such as size- and composition-induced tunable emission, high quantum yield, and low photobleaching
Aureobasidium pulluans was used for fluorescent markers and long-term-distance imaged by the present silicon carbide (SiC) QDs
The culture conditions and method were listed like: 1) the strain is stored in a fungus incubator on the slant, scraped for two loops, and inoculated in sterilized seed media under sterile conditions for reactivation in a thermostatic shaker at 28 ̊C and 200 rpm on a thermostatic shaker for two days; 2) the amount of the activated strain with 8% to mould fermentation medium with 10% of the SiC QDs solution was inoculated and cultured at 28 ̊C and 200 rpm on a thermostatic shaker, and 3) the solution of Aureobasidium pulluans cultured for 2 d and 7 d were dropped onto slides and observed under a fluorescence microscope
Summary
Quantum dots (QDs) have attracted considerable interest over the past two decades due to their remarkable luminescent properties which are known to offer several unique advantages such as size- and composition-induced tunable emission, high quantum yield, and low photobleaching. Recent research results indicated that the widely used II-VI semiconductor QDs, e.g. CdSe, CdTe, CdS, and ZnSe and III-V, e.g. InP and InAs, were found to be cytotoxic to living cells through the release of free metallic cadmium ions and arsenics, even if a protective shell ZnS or a polymer on its surface were systematically and carefully added. It is just the one of the major limiting factors for the applications of II-VI and III-V QDs in efficient living cell imaging because of their cytotoxicity strongly influencing biological cell functioning [5] [6]. Hydrophilic QDs with excellent luminescent properties of biocompatible materials without this problem become important and urgent tasks
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
