Abstract
Fluorescence imaging at longer wavelength and autofluorescence-free imaging were two main strategies to improve the optical imaging performance of traditional near-infrared (NIR) fluorescence imaging, and the design of corresponding imaging agents were heavily explored recently. Herein, polymer-blend dots (PBdots) with strong fluorescence and afterglow luminescence in both NIR-I (700–900 nm) and NIR-II (900–1700 nm) windows were successfully fabricated by encapsulating two functional semiconducting polymers into one nanoparticle. With efficient intra-particle interactions, the PBdots exhibited an afterglow lifetime of 181 s after 730 nm NIR laser activation, which can be activated in vivo. The obtained PBdots with similar emission intensities in NIR-I and NIR-II regions enable fair comparison between different optical imaging modalities, which provides a clear guidance for the choice of suitable imaging modality for different purpose. As the first imaging agents which integrate NIR-II fluorescence imaging and NIR-I afterglow imaging, the PBdots also provide both high resolution and large signal-to-background ratio for blood vessel, lymph node and tumor imaging in living mice.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.