Fabrication of polycaprolactone/heparinized nano fluorohydroxyapatite scaffold for bone tissue engineering uses

Abstract

In this study, some polycaprolactone (PCL)/heparinized nano fluorohydroxyapatite (H-nFHA) scaffolds were fabricated with different amounts of H-nFHA using solid-liquid phase separation and freeze-drying methods. The scaffolds were characterized using Fourier transform infrared spectroscopy, x-ray diffraction, scanning electron microscopy techniques, and compressive test. Their biological behavior also was studied using Alkaline phosphatase activity and MTT assay. The results showed that adding H-nFHA to the PCL matrix improves MG-63 cells adhesion and proliferation and they also showed that heparinizing the nFHA particles improves the mechanical properties and dispersion of the nFHA particles in the PCL matrix for bone tissue engineering uses.