Abstract
Recently, bio-based electrospun nanofiber mats (ENMs) have gained substantial attention for preparing polymer-based biomaterials intended for use in cell culture. Herein, we prepared poly(ethylene-glycol 1,4-Cyclohexane dimethylene-isosorbide-terephthalate) (PEICT) ENMs using the electrospinning technique. Cell adhesion and cell viability of PEICT ENMs were checked by fibroblast cell culture. Field emission electron microscope (FE-SEM) image showed a randomly interconnected fiber network, smooth morphology, and cell adhesion on PEICT ENM. Fibroblasts were cultured in an adopted cell culturing environment on the surface of PEICT ENMs to confirm their biocompatibility and cell viability. Additionally, the chemical structure of PEICT ENM was checked under Fourier-transform infrared (FTIR) spectroscopy and the results were supported by -ray photoelectron (XPS) spectroscopy. The water contact angle (WCA) test showed the hydrophobic behavior of PEICT ENMs in parallel to good fibroblast cell adhesion. Hence, the results confirmed that PEICT ENMs can be potentially utilized as a biomaterial.
Highlights
Bio-based polyesters have been widely studied for biomedical applications such as cell culture, drug delivery, and wound dressing [1,2,3,4,5,6]
The wettability of PEICT Electrospun nanofiber mats (ENMs) was checked by the water contact angle (WCA) test with respect to time as one of the important factors for cell adhesion to optimize the initial resting of fibroblast cells on PEICT ENMs
The in vitro cytocompatibility of PEICT ENMs was analyzed by NIH 3T3 mouse fibroblast cells, Dulbecco’s modified Eagle’s medium (DMEM) was used with 10% fetal bovine serum (FBS) incubated at 37 ◦C with 5% CO2
Summary
Bio-based polyesters have been widely studied for biomedical applications such as cell culture, drug delivery, and wound dressing [1,2,3,4,5,6]. Poly(ethylene-glycol 1,4-Cyclohexane dimethylene-isosorbide-terephthalate) (PEICT) ENMs, in light of their high glass transition temperature [23], good mechanical properties [8], flexibility [6], thermal stability [4], and good crystallinity [23] have potentially been studied for various biomedical applications [4,6]. The wettability of PEICT ENMs was checked by the WCA test with respect to time as one of the important factors for cell adhesion to optimize the initial resting of fibroblast cells on PEICT ENMs. The nanofiber network and surface topography of PEICT ENMs were studied with respect to fibroblast cell infiltration. The results revealed the potential of PEICT ENM to be used as a biomaterial for fibroblast cell culture
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