Abstract
A novel, sensitive and simple amperometric biosensor for the determination of catechol and azinphos methyl based on Tyrosinase enzyme inhibition has been developed. The biosensor was prepared from iridium (IV) oxide, Poly (3,4 ethylenedioxythiophene) nanocomposite, and Tyrosinase immobilized by crosslinking with glutaraldehyde in this nanocomposite platform. Biosensor was successfully used for the dual determination of catechol and azinphos methyl using fixed potential amperometry. Biosensor performance was optimized in terms of iridium (IV) oxide, Poly (3,4 ethylenedioxythiophene) and Tyrosinase. The surface morphology of the enzyme electrode was characterized by scanning electrochemical microscopy, atomic force microscopy, cyclic voltammetry and electrochemical impedance. Under optimized conditions, linear relationships were achieved in the range 0.05−10.65 μM for catechol and 10−200 μM for azinphos methyl. Limit of detection was found as 0.017 μM for catechol and 2.964 μM for azinphos methyl with acceptable repeatability and reproducibility. Finally, developed biosensor was successfully applied to the detection of azinphos methyl tap water, waste water, well water, human urine, serum and saliva.
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