Abstract
Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4-methoxycarbonylphenyl)-porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.
Highlights
Reactive oxygen species (ROS) have been a subject of the extensive studies due to their broad applications such as photodynamic therapy (PDT), photovoltaic device, light harvest coating, and photocatalyst [1, 2]
We report a fabrication of the photofunctional nanoporous alumina membrane (PNAM) and its photophysical property
It has successfully been demonstrated that the singlet oxygen of highly oxidative species is generated from PNAM which is fabricated by the surface modification of Nanoporous alumina membrane (NAM) with organosilane agents, APTES, and the photosensitizer of PtCP
Summary
Reactive oxygen species (ROS) have been a subject of the extensive studies due to their broad applications such as photodynamic therapy (PDT), photovoltaic device, light harvest coating, and photocatalyst [1, 2]. The environmental applications require the high efficiency of ROS generation along with repeatability, the prevention of secondary contamination, and the easy recycling. By the fixation of photosensitizers onto zeolite or MCM-41, thermal stability and handling advantage could be achieved. Both porous materials have a powder like property and a few nanometers of very small pore diameter which disturbs the good contact between the ROS and the target systems [6]. The nanoporous membranes can provide the good contacts between the ROS and the target, and the easy handling advantage and recycling together due to their advantages of pore diameter control, unidirectional ordered pore direction, and free-standing nature of membrane
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