Fabrication of osteogenesis induced WE43 Mg-Hydroxyapatite composites with low biodegradability and increased biocompatibility for orthopedic implant applications

Abstract

Good biocompatibility, low biodegradation rate and excellent mechanical properties are essential for desirable performance of metallic biomaterials during osseointegration process. In the present study, bioabsorbable WE43 Mg alloy was used for fabrication of bio-composites with addition of nanosized hydroxyapatite (HA) particles by means of multi pass friction stir processing (FSP) for orthopedic implant applications. Microstructure, in vitro biodegradation and biocompatibility, wettability, and microhardness of resultant bio-composites were characterized. Results showed that applying severe plastic deformation through FSP caused significant grain refinement of Mg matrix and homogenous dispersion of fragmented secondary phase particles. Moreover, applying higher number of FSP passes increased uniformity of distribution of HA nanoparticles throughout Mg matrix. Compared to coarse grain samples, grain refined samples showed lower Mg2+ concentration and H2 evolution in simulated body fluid (SBF). Moreover, addition of HA into Mg matrix, reduced Mg2+ release and H2 evolution. The bio-composites illustrated uniformly and flatly corroded surface compared to samples without HA nanoparticles. During immersion of the bio-composites in SBF solution, a cauliflower structure of Ca–P compounds deposited on the surface of bio-composites which confirmed acceptable biomineralization. Contact angle measurement demonstrated that wettability of fabricated bio-composites increased by increasing number of FSP passes. Mouse osteoblast MC3T3 cell culture indicated that the fabricated bio-composites with uniform distribution of HA nanoparticles showed excellent in vitro biocompatibility, cell viability, and cell proliferation. Eventually, it was found that applying further FSP and presence of HA nanoparticles resulted in increased microhardness which is an indication of higher load-bearing capability of the fabricated bone implants.