Fabrication of multifunctional shell cross-linked micelles for targeting drug release

Abstract

Thermosensitive amphiphilic poly(N-acroyloxysuccinimide)-b-poly(N-isopropylacrylamide)-b-poly(e-caprolactone) triblock copolymer was synthesized via the combination of reversible addition fragmentation chain transfer and ring-opening polymerization techniques. Shell cross-linked micelle (SCL) was further developed by the addition of cystamine as a di-functional cross-linker into the micellar solution. The persistence of regularly spherical shape against media change demonstrated locked micellar structure resulting from sufficient shell cross-linking. The lower critical solution temperature of the resulting SCL micelles was around 38 °C. The in vitro drug release study was carried out to illustrate the temperature-responsive drug release behaviors. To enhance the internalization to tumor cells, transferring (Tf) was further conjugated to the SCL micelles, and endocytosis experiments further confirmed the efficient uptake of Tf-SCL micelles by tumor cells, indicating that the Tf-SCL micelles would be a promising candidate for tumor-targeted drug delivery.