Abstract
The development of high-performance protein-imprinted materials for adsorption and separation to obtain high-purity, high-quality biomolecules is still one of the most important techniques to promote the rapid development of the protein drug industry. In this paper, a novel highly specific bovine serum albumin (BSA) cobalt–molybdenum double-hydroxides surface-imprinted composite particles (Co–Mo LDHs@MIPs) were constructed by the surface imprinting technique combined with metal chelation. ZIF-67 was etched by molybdate ions to obtain unique MOF-derived bimetallic hydroxide particles (Co–Mo LDHs), which were used as the carrier. The rough outer surface and bimetallic ion coordination of Co–Mo LDHs enable anchoring more template proteins. The adsorption capacity of Co–Mo LDHs@MIPs was up to 423.37 mg/g for BSA in 60 min with an imprinting factor of 2.65. In addition, Co–Mo LDHs@MIPs can selectively recognize and separate BSA from mixed proteins and fetal bovine serum and exhibit high reusability stability.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.