Fabrication of Metal Ion–BSA Chelation-Targeted Surface-Imprinted Particles for Protein Separation

Abstract

The development of high-performance protein-imprinted materials for adsorption and separation to obtain high-purity, high-quality biomolecules is still one of the most important techniques to promote the rapid development of the protein drug industry. In this paper, a novel highly specific bovine serum albumin (BSA) cobalt–molybdenum double-hydroxides surface-imprinted composite particles (Co–Mo LDHs@MIPs) were constructed by the surface imprinting technique combined with metal chelation. ZIF-67 was etched by molybdate ions to obtain unique MOF-derived bimetallic hydroxide particles (Co–Mo LDHs), which were used as the carrier. The rough outer surface and bimetallic ion coordination of Co–Mo LDHs enable anchoring more template proteins. The adsorption capacity of Co–Mo LDHs@MIPs was up to 423.37 mg/g for BSA in 60 min with an imprinting factor of 2.65. In addition, Co–Mo LDHs@MIPs can selectively recognize and separate BSA from mixed proteins and fetal bovine serum and exhibit high reusability stability.