Abstract
Inorganic hydroxyapatite nanoparticles (HANPs) and two kinds of organic biomolecules (i.e., fluorescent dye rhodamine 6G and protein lysozyme) were coencapsulated into alginate microspheres through an air dynamical atomization with optimized operation conditions. The synthesized microspheres have several advantages: HANP provides osteoconductivity and mechanical strength, rhodamine 6G (R6G) and lysozyme act as model drugs, and alginate provides excellent biocompatibility and carboxylate functionality. The results of fluorescent microscopic images indicated the successful dual encapsulation of HANPs and lysozyme inside the alginate microspheres. Furthermore, the results of 3- (4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay showed that the fabricated alginate microspheres could be uptaken by HepG2 without apparent cytotoxicity. The dual encapsulated alginate microspheres fabricated in this study show great potential in many biomedical applications.
Highlights
Microsphere is one of the most commonly used morphologies for alginate in practical applications
The synthesized microspheres have several advantages: hydroxyapatite nanoparticles (HANPs) provides osteoconductivity and mechanical strength, rhodamine 6G (R6G) and lysozyme act as model drugs, and alginate provides excellent biocompatibility and carboxylate functionality
The dual encapsulated alginate microspheres fabricated in this study show great potential in many biomedical applications
Summary
Microsphere is one of the most commonly used morphologies for alginate in practical applications. When the alginate droplet gets into contact with calcium ions in solution, the outermost surface of the alginate would immediately interact with calcium ions (i.e., gelation) and form spherical morphology. Pipette and syringe are commonly used to synthesize alginate spheres with very uniform particle size.. Pipette and syringe are commonly used to synthesize alginate spheres with very uniform particle size.7–10 Membrane emulsification is another popular method to form spherical morphology.. We and others have utilized atomization equipped with air-flow or an external electric field to make alginate microspheres.. The particle size could be controlled by the strength of external electric field and the flow rate of the alginate solution We and others have utilized atomization equipped with air-flow or an external electric field to make alginate microspheres. The particle size could be controlled by the strength of external electric field and the flow rate of the alginate solution
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