Fabrication of Gelatin-Based Electrospun Composite Fibers for Anti-Bacterial Properties and Protein Adsorption.

Yingbo Wang,Wenguo Cui,Yimin Wang,Ya Gao

doi:10.3390/md14100192

Abstract

A major goal of biomimetics is the development of chemical compositions and structures that simulate the extracellular matrix. In this study, gelatin-based electrospun composite fibrous membranes were prepared by electrospinning to generate bone scaffold materials. The gelatin-based multicomponent composite fibers were fabricated using co-electrospinning, and the composite fibers of chitosan (CS), gelatin (Gel), hydroxyapatite (HA), and graphene oxide (GO) were successfully fabricated for multi-function characteristics of biomimetic scaffolds. The effect of component concentration on composite fiber morphology, antibacterial properties, and protein adsorption were investigated. Composite fibers exhibited effective antibacterial activity against Staphylococcus aureus and Escherichia coli. The study observed that the composite fibers have higher adsorption capacities of bovine serum albumin (BSA) at pH 5.32–6.00 than at pH 3.90–4.50 or 7.35. The protein adsorption on the surface of the composite fiber increased as the initial BSA concentration increased. The surface of the composite reached adsorption equilibrium at 20 min. These results have specific applications for the development of bone scaffold materials, and broad implications in the field of tissue engineering.

Highlights

Human bones are composed of organic and inorganic components, hydroxyapatite (HA) and collagen
When investigating the effect of initial concentration on protein adsorption, the results demonstrated that Gel/CS/HA/graphene oxide (GO) composite fibers have good protein adsorption performance
We demonstrated that quaternary Gel/CS/HA/GO composite fibers could be prepared by a simple electrospinning method

Summary

Introduction

Human bones are composed of organic and inorganic components, hydroxyapatite (HA) and collagen. Composite materials that mimic the bone matrix have important clinical applications. HA exhibits excellent biocompatibility and biodegradability and, is a high-profile artificial bone material. Its insufficient flexural and compressive strength and high brittleness limit its medical applications [1]. Gelatin (Gel) is a modified collagen product and a natural polymer; it is structurally similar to collagen in extracellular matrices [2,3,4]. These composite materials can adapt well to the internal environment of humans [5].

Objectives

Discussion

Conclusion