Fabrication of flexible paper-based conducting molecularly imprinted polymer as analytical devices: Electrochemically assisted solid phase microextraction and selective flexible sensor for determination of naproxen

Abstract

An electrochemical method is presented for the preparation of paper-based conductive molecular imprinted polymer (CMIP) film as a recognition element of a sensor and a selective absorber in separation methods. The paper became conductive by chemically incorporating polypyrrole (PPy) doped with anion dopant. The effects of the anion dopant and oxidant type on the conductivity of the modified paper were studied. Afterward, a CMIP film based on PPy and an anionic drug as a template was electrochemically synthesized on conductive paper surface, which was then utilized as a selective sorbent for electrochemically assisted solid phase microextraction (EC-SPME) and a recognition element in fabricating flexible drug-selective sensor. Factors affecting the performance of the paper-based EC-SPME device were studied in naproxen (NAP) analysis. All quantitative analytical parameters in the EC-SPME device were obtained by UV–vis absorption spectroscopy. Under the optimal conditions, linearity was achieved within the range of 2.0 × 10−7–2.0 × 10−5 mol/L with determination coefficient (R2 = 0.99) and the limit of detection (LOD) was 0.6 × 10−7mol/L. Moreover, a fabricated paper-based NAP-selective sensor, showed Nernstian response slopes of −61.6 ± 0.5 mV decade−1 over the linear range of 4.0 × 10−7–1.0 × 10−2 mol/L and LOD was 2.0 × 10−7 mol/L. The applicability and efficiency of the paper-based analytical devices were investigated for NAP determination as a model drug in human serum samples. The recoveries were obtained in the range of 90.0–103.0 %. Accordingly, paper-based CMIPs were found to be a novel fabrication method for efficient, selective, flexible and cost-effective material for analytical devices.