Abstract
Composite and macroporous gel beads for the immobilization of enzymes were produced by combining suspension gelation with sedimentation polymerization. Physically cross-linked calcium alginate microcapsules containing lipase were prepared by electrostatic atomization. Monodisperse millimeter-sized poly(ethylene glycol) diacrylate hydrogel (composite gel) beads were fabricated by polymerizing droplets of pre-gel aqueous suspension that contained microcapsules during their descent in silicone oil. Macroporous gel beads with entrapped lipase were prepared by treating the composite gel beads with a trisodium citrate aqueous solution. The lipase immobilized within the composite and macroporous gels successfully catalyzed the hydrolysis of p-nitrophenyl acetate over successive cycles of use. The kinetics of the enzymatic reaction was analyzed using the Michaelis–Menten equation.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
