Abstract
Numerous wound care products have been investigated for skin tissue engineering. Factors which influence to skin substitute selection are condition of patient, wound depth, infection in wound. Other factors which also play role in deciding skin substitute are cost, its availability, ease of storage, required operative interventions. With perspective of Indian market, commercial available skin substitutes are either costly or their availability restricted to major cities only. A cost effective skin substitute is strongly needed to heal wounds with minimal scarring and maximum function. The aim of this study was to investigate the possibility of synthetic scaffold loaded with Wharton’s jelly derived Mesenchymal stem cells and to access the role of scaffolds in proliferation and differentiation of MSCs in-vitro, in order to achieve for the healing of wound graft substitutes with improved biological properties. As a result, WJ-MSCs were isolated, harvested and seeded on the surface of the fabricated PCL/GE nanofibrous scaffold. The biological properties and growth of MSCs were studied for anti-inflammation, cytotoxicity, cell proliferation, and SEM analysis indicated that the fabricated synthetic scaffold supported cells attachment, viability, and proliferation of cells. The characterization studies of nanofibers were studied for ATR-FTIR, XRD, TEM, viscosity, and degradation studies suggest that the nanofibrous scaffold loaded with stem cells could be an excellent tissue-engineered skin base for wound healing and skin regeneration.
Highlights
Skin wounds continue to be a major public health concern worldwide resulting from surgical procedures, reduced circulations, mechanical trauma, burns, or aging [1]
This study investigates the potential and future prospects of synthetic scaffolds as skin substitutes fabricated using electrospinning for tissue repair and regeneration
A major constrain to stem cell therapeutic to clinical application is the supply of adequate number of cells [34]. 3D scaffolds provide a better resemblance of natural environment which can hardly be represented by 2D plastic substrate [35]
Summary
Skin wounds continue to be a major public health concern worldwide resulting from surgical procedures, reduced circulations, mechanical trauma, burns, or aging [1]. Among all the injuries burn is a major global public health problem and a main cause of morbidity [2, 3]. Several natural skin substitutes such as allografts, xenografts and autografts have been widely used to restore wound defects. These skin substitutes cannot achieve skin regeneration due to risk of infection, limited donor sites, slow healing and lead formation of scar [4, 5]. The rapid growth of nanotechnology has spurred the progression of nanofibrous scaffolds which has been found to play significant role in the management of skin-related disorders and deep burn [6]
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