Abstract
Serum 25-hydroxyvitamin D (25(OH)D) has been clinically considered as a novel biomarker for vitamin D deficiency. The current standard technologies for the detection of 25(OH)D are performed in sophisticated laboratories exhibiting the practical limitations for onsite and affordable testing. Therefore, the development of a cost-effective device for Vitamin D is extremely necessary to provide an earlier diagnosis. Herein, for the first time, we propose a novel label-free impedimetric immunosensor for the detection and quantification of 25-hydroxyvitamin D3 (25(OH)D3) biomarker in serum samples based on the Au nanoparticles functionalized GCN–β-CD nanocomposite. To fabricate the sensing probe, Ab-25(OH)D3 antibodies were covalently immobilized on GCN–β-CD@Au/GCE using carbodiimide chemistry. The surface morphology and structural properties of constructed immunosensor were confirmed by different analytical techniques. Electrochemical impedance spectroscopy technique (EIS) has been selected as the main detection method to measure the Antibody (Ab) and Antigen (Ag) interaction at the immunosensor surface because it is label-free, less destructive to the activities of the biomolecule, and highly sensitive. The as-prepared immunosensor exhibited an excellent concentration range from 0.1 ng/ml to 500 ng/ml with the lowest limit of detection of 0.01 ng/ml. Furthermore, the sensing probe was validated in serum samples and obtained results were compared with the standard CLIA technique. The results have revealed that the sensing probe could be used for clinical diagnosis of Vitamin D deficiency in the clinical laboratories.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.