Abstract
AbstractIn this study, polycaprolactone/mineral trioxide aggregate (PCL/MTA) scaffolds were successfully fabricated via an electrohydrodynamic jet (or E-jet) 3D printing system developed by our group. The viscosity of the composited solutions and the key process parameters (i.e. applied voltage and feed rate) were investigated to achieve an optimal process condition. To investigate the potential of PCL/MTA scaffolds to support regeneration ability for dentin related tissue, we seeded dental pulp stem cells on the scaffolds, and compared the results with cell-seeded PCL scaffolds. Assessment of cell viability and proliferation using live/dead cell staining and MTS assay showed compatibility of PCL and PCL/MTA scaffolds for cellular attachment and growth. These scaffolds could be used for fabrication of three-dimensional tissues and in future could be applied to dentin and periodontal tissue engineering applications.
Highlights
Dentin, a hard mineralised avascular connective tissue that forms the bulk of the tooth structure, is supported by the pulp
Solution viscosities The viscosity measurement was conducted to investigate how the addition of Mineral Trioxide Aggregate (MTA) affected the viscosity of PCL solution
Solution viscosity is one of the key process parameters of E-jetting (Huang et al, 2013), which is effected by the material concentration
Summary
A hard mineralised avascular connective tissue that forms the bulk of the tooth structure, is supported by the pulp. The odontoblasts contained in dentin are derived from dental pulp stem cells (DPSCs). DPSCs have the ability to differentiate into odontoblast-like cells (Scheller, Krebsbach, & Kohn, 2009). Periodontal disease is known as gum disease. It can be from a minor gum inflammation to serious disease that results in major damage of the tissue and bone that supports the teeth. When gum diseases are left untreated, it causes inflammation around the tooth. The tissue that supports the teeth gets destroyed and the tooth may become loose and have to be removed. Tissue engineered dentin scaffolds have been intensively studied (Bohl, Shon, Rutherford, & Mooney, 1998; Duailibi et al, 2004; Young et al, 2002), which aimed at dentin tissue regeneration
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
