Fabrication of Copper Nano/Honey Loaded Polymeric Composite with Microbial activity

Abstract

Polymers are known as lead compounds for therapeutic drug development to treat a variety of conditions. Polymers have become an essential part of drug delivery systems because of their improved pharmacokinetic properties. Additionally, Copper is also recognized as a well-known antibacterial metal. In an in-vitro assay, solid copper surfaces killed 99.9% of microbes within 2hours of contact. Honey exhibits a broad-spectrum antibacterial activity against gram-positive and gram-negative bacteria. It also has anti-inflammatory and antioxidant activities. In this study, polyvinyl alcohol (PVA) and starch (St) are prepared by cross-linking with glutaraldehyde (GA) composite sheets and filled with pure honey and copper nanoparticles. The synthesized films are characterized by various methods such as X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Chemical analysis is also carried out such as Swelling study and Expansion study. Copper nanoparticles are characterized by ultraviolet (UV)-visible spectroscopy and dynamic light scattering analysis (DLS). Along with this, Honey is characterized by ultraviolet (UV)-visible spectroscopy. Various network parameters were evaluated using the source data. Swelling parameters were also evaluated using a gelatin swelling assay. The antibacterial activity of PVA/St films loaded with honey and copper nanoparticles was tested against Escherichia coli bacteria (E. Coli bacteria).